Saliva Profile III — Comprehensive Hormone & 4-Point Cortisol Panel (E2, Pg, T, DS & Cx4)
Saliva Profile III is the most comprehensive saliva hormone panel in the series, providing a complete assessment of sex hormones AND a 4-point diurnal cortisol rhythm. Measuring estradiol (E2), progesterone (Pg), testosterone (T), DHEA-S (DS), and cortisol at four time points throughout the day, this panel gives you and your practitioner the most complete picture of hormonal and adrenal function available.
What's Measured

Estradiol (E2) — primary estrogen for reproductive, bone, cardiovascular, and mood health
Progesterone (Pg) — estrogen balance, sleep quality, and uterine health
Testosterone (T) — libido, energy, lean mass, and cognitive drive
DHEA-S (DS) — adrenal androgen for energy, immunity, and anti-aging
Cortisol x4 (Cx4) — four timed measurements mapping your complete daily cortisol curve

Who Needs Profile III?
This is the most informative panel for anyone with significant hormonal AND adrenal symptoms. It is recommended for those with perimenopause, menopause, PMS, PCOS, adrenal fatigue, chronic fatigue, thyroid issues compounded by adrenal stress, or anyone undergoing comprehensive hormonal optimization. Results reviewed by Michelle Tonkin ND with a full personalized protocol.
Frequently Asked Questions
Q: Why is 4-point cortisol the gold standard?
A: Cortisol levels fluctuate significantly throughout the day. Four measurements (morning, noon, afternoon, evening) reveal the full diurnal rhythm — identifying patterns like high evening cortisol, flat patterns, or inverted curves that a single measurement cannot detect.
Q: Is this test appropriate for both men and women?
A: Yes — while primarily used for women with hormonal imbalances, men can also benefit from this panel to assess testosterone, DHEA, and adrenal function comprehensively.