Saliva Profile II — Advanced Hormone Panel with 2-Point Cortisol (E2, Pg, T, DS & Cx2)
Saliva Profile II builds on the foundational Profile I by adding a 2-point cortisol measurement for deeper adrenal assessment. This panel measures estradiol (E2), progesterone (Pg), testosterone (T), DHEA-S (DS), and cortisol at two time points — providing a more complete picture of hormonal balance and HPA axis function than a single cortisol measurement alone.
What's Measured

Estradiol (E2) — reproductive health, bone density, mood, and cardiovascular protection
Progesterone (Pg) — balances estrogen, supports sleep quality and anxiety
Testosterone (T) — energy, libido, lean mass, and mental drive
DHEA-S (DS) — adrenal androgen supporting energy and immune health
Cortisol x2 (Cx2) — morning and evening cortisol to assess adrenal rhythm patterns

Who Should Choose Profile II?
Profile II is recommended for individuals who want more adrenal insight than the single-point cortisol in Profile I provides, but don't yet need the full 4-point assessment. It's particularly useful for people experiencing sleep disruption, afternoon energy crashes, anxiety, or suspected adrenal rhythm imbalances alongside hormonal symptoms. Results reviewed by Michelle Tonkin ND.
Frequently Asked Questions
Q: What additional information does the 2-point cortisol provide?
A: By measuring cortisol at both morning and evening, Profile II can detect whether your cortisol is elevated at night (causing insomnia) or fails to drop appropriately — common patterns in chronic stress and burnout.
Q: Should I choose Profile II or Profile III?
A: If you have significant adrenal symptoms (fatigue, insomnia, anxiety, blood sugar swings), Profile III (4-point cortisol) provides a more complete picture. Profile II is an excellent intermediate option for most hormonal health assessments.