Adrenal Stress Profile (DS & Cx4) — Professional Adrenal Function & Cortisol Rhythm Testing
The Adrenal Stress Profile (DS & Cx4) is a professional-grade hormone assessment using saliva collection to measure DHEA-S and a 4-point cortisol rhythm throughout the day. This panel provides a detailed picture of adrenal gland function, HPA axis regulation, and cortisol output patterns — giving you and your practitioner actionable data for addressing adrenal fatigue, burnout, and chronic stress.
What's Measured

DHEA-S (DS) — adrenal androgen supporting energy, immune function, mood, and anti-aging
Cortisol x4 (Cx4) — four timed cortisol measurements (morning, noon, afternoon, evening) mapping your full daily cortisol rhythm

Why 4-Point Cortisol Testing?
A single cortisol reading misses critical information about your adrenal rhythm. The 4-point (diurnal) cortisol test reveals whether your cortisol peaks appropriately in the morning, dips at noon, maintains a healthy afternoon level, and drops at night — or whether you have patterns like flat cortisol, inverted rhythms, or evening spikes that disrupt sleep. This comprehensive view is essential for accurately diagnosing adrenal dysfunction and tailoring a treatment protocol.
Who Needs This Test?
This profile is ideal for individuals experiencing chronic fatigue, burnout, insomnia, anxiety, poor stress tolerance, blood sugar dysregulation, or immune challenges. Results reviewed by Michelle Tonkin ND with a personalized protocol for adrenal restoration.
Frequently Asked Questions
Q: Is saliva testing accurate for cortisol?
A: Yes — saliva cortisol testing is the gold standard for assessing free (bioavailable) cortisol and is widely used in research and clinical practice for HPA axis evaluation.
Q: How do I prepare for the test?
A: Avoid eating, drinking, brushing teeth, or using mouthwash for 30 minutes before each saliva collection. Instructions are included with your test kit.